Air bubble example
In the process of cover slipping, air bubbles can occur. This happens when air gets trapped in between the slide and coverslip. Here are a few tips for creating slides without bubbles and advancing your slide turn around time.
1- Take the slide out of the alcohol cup and quickly wipe the bottom and sides of the slide on a paper towel to remove excess alcohol.
2- Hold the slide lengthwise with 2 fingers
3- Apply 2-3 drops of cytoseal
4-Tilt slide at 45 degree angle towards you
5-Apply coverslip from bottom of slide and slowly ease coverslip down to avoid air bubbles
6-If bubbles still occur, squeeze them out applying light pressure with forceps to the coverslip and draw the bubbles to the end of the slide.
7- If you need to redo the slide, discard coverslip, re-dip slide in alcohol and repeat steps.
These tips should help decrease air bubbles. Practice is key and in no time you'll be making great slides!
1- Take the slide out of the alcohol cup and quickly wipe the bottom and sides of the slide on a paper towel to remove excess alcohol.
2- Hold the slide lengthwise with 2 fingers
3- Apply 2-3 drops of cytoseal
4-Tilt slide at 45 degree angle towards you
5-Apply coverslip from bottom of slide and slowly ease coverslip down to avoid air bubbles
6-If bubbles still occur, squeeze them out applying light pressure with forceps to the coverslip and draw the bubbles to the end of the slide.
7- If you need to redo the slide, discard coverslip, re-dip slide in alcohol and repeat steps.
These tips should help decrease air bubbles. Practice is key and in no time you'll be making great slides!
Mobile Mohs Inc.
WE KNOW MOHS
No comments:
Post a Comment